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Supplementary data

Multiple acquisitions of CTX-M plasmids in the rare D₂ genotype of Escherichia coli provide evidence for convergent evolution, by C. Deschamps, O. Clermont, M. C. Hipeaux, G. Arlet, E. Denamur and C. Branger

Microbiology vol. 155, part 5, pp. 1656-1668

Table S1. Oligonucleotides used for PCR amplification and DNA sequencing of the region surrounding TSPE4.C2 bordered by yiiD and yiiE.

Fig S1. Population genetics analysis based on MLST data. (a) Table listing the strains included in the MLST analysis. The STs were attributed from the combination of allelic variations of six housekeeping genes (trpA, trpB, pabB, putP, icd and polB). For each strain, the genotype, CTX-M production and the phylogenetic D subgroup are indicated. (b) Minimum spanning tree analysis of the A, B1, B2 and D phylogenetic group strains constructed from the STs with Prim's algorithm (www.pubmlst.org/analysis). The number within each circle identifies an ST. The length of the branch represents the genetic distance between the two neighbouring STs. The D subgroups of genetically related isolates are numbered I to IX and visualized as dotted ellipses. STs including D₂ genotype strains are shown in grey. The grouping pattern obtained is consistent with that obtained by phylogenetic analysis (see Fig. 1 in paper).

Fig S2. Schematic representation of the genetic environment of bla_CTX-M genes from the 18 ESBL-producing D₂ E. coli isolates studied (see Table 2 in paper). Representation is as described in the paper by Eckert et al. (2006).

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