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In the non-insect-transmissible line of onion yellows phytoplasma (OY-NIM), the plasmid-encoded transmembrane protein ORF3 lacks the major promoter region, by Y. Ishii, S. Kakizawa, A. Hoshi, K. Maejima, S. Kagiwada, Y. Yamaji, K. Oshima and S. Namba

Microbiology vol. 155, part 6, pp. 2058 - 2067

Fig. S1. SDS-PAGE analysis of overexpressed and purified ORF3. Lane 1, molecular markers; lane 2, purified ORF3. The arrowhead indicates the band of ORF3 protein. [PDF] (79 kb)

Fig. S2. Transcriptional analysis of EcOYM-encoded genes. RT-PCR analysis of EcOYM-encoded genes (orf1-5) was performed using total RNA isolated from OY-M-infected insects. The lengths of orf1-4 are 486, 603, 465 and 315 bp, respectively. The full-length of orf5 is 1305 bp, but only a partial region was amplified. To prove an absence of DNA contamination in the total RNA, PCR analysis of the OY-M amp gene was performed without reverse transcription (negative control). [PDF] (84 kb)

Fig. S3. Complete set of EcOY-DNAs and pOY plasmids from OY lines. orf3 is encoded on EcOYNIM but not on pOYNIM. The major promoter of orf3 (ORF3-pro2) is indicated by the open arrows on EcOYW and EcOYM, but it is not encoded on EcOYNIM. [PDF] (88 kb)

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef