Function of the N-terminal region of the phosphate-sensing histidine kinase, SphS, in Synechocystis sp. PCC 6803, by S. Kimura, Y. Shiraiwa and I. Suzuki
Microbiology vol. 155, part 7, pp. 2256 - 2264
Table S1. Primers used in this study [PDF] (75 kb)
Fig. S1. Construction of sphS genes lacking the N-terminal regions. Details are described in Methods. The numbers in parentheses correspond to the numbers of primers in Table S1 that were used for amplification of DNA fragments by PCR. [PDF] (186 kb)
Fig. S2. Construction of sphS genes whose hydrophobic region was replaced with that of NrsS, a histidine kinase responding to nickel ions. Details are described in the Methods section of the main paper. The numbers in parentheses correspond to the numbers of primers in Supplementary Table S1 that were used for amplification of DNA fragments by PCR. [PDF] (98 kb)
Fig. S3. Construction of sphS genes possessing a single amino acid substitution in the PAS domain (a) or His-tagged sphS (b). Details are included in the Methods section of the main paper. The numbers in parentheses correspond to the numbers of primers in Supplementary Table S1 that were used for amplification of DNA fragments by PCR. [PDF] (117 kb)
Fig. S4. Construction of plasmids for overexpressing the sphS or modified sphS genes. Details are provided in the Methods section of the main paper. The numbers in parentheses correspond to the numbers of primer in Supplementary Table S1 that were used for amplification of DNA fragments by PCR. [PDF] (108 kb)
Fig. S5. Amino acid alignment of hydrophobic regions from SphS and NrsS. Hydrophobic regions of SphS and NrsS were determined by the prediction program TMpred (http://www.ch.embnet.org/software/TMPRED_form.html). The amino acid alignment was created by CLUSTAL W. [PDF] (75 kb)
Copyright © 2009 Society for General Microbiology.
