HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sun, Y. Articles by Haydock, S. F. Search for Related Content PubMed Articles by Sun, Y. Articles by Haydock, S. F. Agricola Articles by Sun, Y. Articles by Haydock, S. F.

Organization of the biosynthetic gene cluster in Streptomyces sp. DSM 4137 for the novel neuroprotectant polyketide meridamycin

The name of the third author was incorrectly spelt as Markiyan Samboskyy; the correct spelling is Markiyan Samborskyy, as shown in the byline above.

The Methods section DNA sequencing analysis (pages 3509–3510 of the printed article) should read as follows:

DNA sequencing analysis. Automated DNA sequencing was carried on double-stranded DNA templates by the dideoxynucleotide chain-termination method in the University of Cambridge, Department of Biochemistry, DNA sequencing facility. Taq dideoxy terminator sequencing reactions were carried out using the Big Dye Terminator kit v. 3.1 and analysed using an AB 3730XL DNA sequencer according to the manufacturer's protocols.

Cosmid DNA for initial end sequencing was prepared from overnight growth of 1 ml culture in 96-well plates in 2x LB medium containing kanamycin and ampicillin. The complete sequence of the cosmid was obtained by shotgun sequencing using Sau3AI for partial digestion of the parent cosmid. The 2–5 kbp size fraction of this digest was eluted from an agarose gel using the Gene Clean kit (Bio 101, Bio-Rad) and subcloned into the BamHI site of dephosphorylated pSG397 (Hashimoto-Gotoh et al., 1995). These random subclones were sequenced using template DNA obtained from 1 ml cultures grown overnight at 37 °C in 96-well plates, with universal forward and reverse sequencing primers.

PHRED (Ewing et al., 1998; Ewing & Green, 1998), PHRAP (www.phrap.org) and CONSED v14 (Gordon et al., 1998, 2004) were used for sequence basecalling, assembly and editing. The remaining gaps were finished by primer-walking sequencing, using custom-designed oligonucleotide primers with cosmid template. Both strands were completely sequenced with a mean eightfold coverage per base. Database searches were done using BLAST (Altschul et al., 1997).

REFERENCES

Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[Abstract/Free Full Text]

Ewing, B. & Green, P. (1998). Basecalling of automated sequencer traces using PHRED. II. Error probabilities. Genome Res 8, 186–194.[Abstract/Free Full Text]

Ewing, B., Hillier, L., Wendl, M. & Green, P. (1998). Basecalling of automated sequencer traces using PHRED. I. Accuracy assessment. Genome Res 8, 175–185.[Abstract/Free Full Text]

Gordon, D., Abajian, C. & Green, P. (1998). CONSED: a graphical tool for sequence finishing. Genome Res 8, 195–202.[Abstract/Free Full Text]

Gordon, D. (2004). Viewing and editing assembled sequences using CONSED. In Current Protocols in Bioinformatics, pp. 11.2.1–11.2.43. Edited by A. D. Baxevanis & D. B. Davison. New York: Wiley.

Hashimoto-Gotoh, T., Mizuno, T., Ogasahara, Y. & Nakagawa, M. (1995). An oligonucleotide-directed dual amber method for site-directed mutagenesis. Gene 23, 271–275.

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sun, Y. Articles by Haydock, S. F. Search for Related Content PubMed Articles by Sun, Y. Articles by Haydock, S. F. Agricola Articles by Sun, Y. Articles by Haydock, S. F.