Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 151 (2005), 3689-3697; DOI  10.1099/mic.0.28045-0
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Manteca, A.
Right arrow Articles by Sánchez, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Manteca, A.
Right arrow Articles by Sánchez, J.
Agricola
Right arrow Articles by Manteca, A.
Right arrow Articles by Sánchez, J.
Microbiology 151 (2005), 3689-3697; DOI  10.1099/mic.0.28045-0
© 2005 Society for General Microbiology

A death round affecting a young compartmentalized mycelium precedes aerial mycelium dismantling in confluent surface cultures of Streptomyces antibioticus

Ángel Manteca1, Marisol Fernández2 and Jesús Sánchez1

1 Area de Microbiología, Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Julián Clavería s/n, 33006 Oviedo, Spain
2 Laboratorio de Proteómica, Centro Nacional de Biotecnología, Cantoblanco, 28049 Madrid, Spain

Correspondence
Jesús Sánchez
jsm{at}fq.uniovi.es

Development-associated cell-death processes were investigated in detail during the growth and differentiation of Streptomyces antibioticus ATCC 11891 on confluent surface cultures, by using fluorescent viability probes, membrane and activity fluorescence indicators, and electron microscopy analysis. A previously unsuspected complexity was revealed, namely the presence of a very young compartmentalized mycelium that dies following an orderly pattern, leaving alternating live and dead segments in the same hypha. This death round is followed by the growth of a second mycelium which develops rapidly from the live segments of the first mycelium and dies massively in a second death round, which extends over the phases of aerial mycelium formation and sporulation.

Abbreviations: CFDA, carboxyfluorescein diacetate; CLSM, confocal laser-scanning fluorescence microscopy; FM 4-64, N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide; PI, propidium iodide; WGA, wheat germ agglutinin




This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
A. Manteca, R. Alvarez, N. Salazar, P. Yague, and J. Sanchez
Mycelium Differentiation and Antibiotic Production in Submerged Cultures of Streptomyces coelicolor
Appl. Envir. Microbiol., June 15, 2008; 74(12): 3877 - 3886.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2005 Society for General Microbiology.