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Journal of General Microbiology 63 (1970), 211-220; DOI  10.1099/00221287-63-2-211
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A Simple Chemically Defined Medium for the Production of Phase I Bordetella pertussis

D. W. Stainer1 and M. J. Scholte1

Connaught Medical Research Laboratories, University of Toronto, Toronto, Ontario, Canada

ABSTRACT

SUMMARY: A simple, chemically defined medium is described consisting of sodium glutamate, proline, cystine, salts, and growth factors, which is suitable for the large-scale production of phase I Bordetella pertussis. More than 30 x 109 organisms/ml. were produced in 48 to 72 h. growth in shake flasks and fermentors. The cultures were detoxified by the addition of 0.14% formalin to yield vaccines which were non-toxic to mice and guinea pigs and had good mouse-protective antigen levels. The antigenic stability was satisfactory after storage of the final vaccines at elevated temperatures.




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