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Published online ahead of print on 16 April 2009 as doi:10.1099/mic.0.024455-0
Microbiology 2009;155:1669.

Microbiology (2009), DOI 10.1099/mic.0.024455-0
© 2009 Society for General Microbiology
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Microbiology 0 (2009), mic.0.024455; DOI  10.1099/mic.0.024455-0
© 2009 Society for General Microbiology

Identification of the binding site of the {sigma}54 hetero-oligomeric FleQ/FleT activator in the flagellar promoters of Rhodobacter sphaeroides

J. Sánchez-Peña, S. Poggio, U. Flores-Pérez, A. Osorio, C. Domenzain, G. Dreyfus and L. Camarena1

Universidad Nacional Autonoma de Mexico

ABSTRACT

Expression of the flagellar genes in Rhodobacter sphaeroides is dependent on one of the four sigma-54 factors present in this bacterium and on the enhancer binding proteins (EBP) FleQ and FleT. These proteins, in contrast with other well-characterized EBPs, carry out activation as a hetero-oligomeric complex. To further characterize the molecular properties of this complex we mapped the binding sites or UAS (Upstream Activation Sequence) of six different flagellar promoters. In most cases the UAS were identified at approximately 100 bp upstream from the promoter. However, the activity of the divergent promoters flhAp-flgAp which are separated by only 53 bp, is mainly dependent on a UAS located approximately 200 bp downstream from each promoter. Interestingly, a significant amount of activation mediated by the upstream or contralateral UAS was also detected, suggesting that the architecture of this region could be important for the correct regulation of these promoters. Sequence analysis of the regions carrying the potential FleQ/FleT binding sites revealed a conserved motif. In vivo footprinting experiments of the motAp promoter allowed us to identify a protected region that overlaps with this motif. These results allow us to propose a consensus sequence that represents the binding site of the FleQ/FleT activating complex.

1 E-mail: rosal{at}servidor.unam.mx






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Copyright © 2009 Society for General Microbiology.