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Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model

¹ Animal Infectious Disease Laboratory, Yangzhou University;
² College of Clinical Medicine,Yangzhou University

ABSTRACT

Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) establish infections in extraintestinal habitats of different hosts. As diversity, epidemiological sources, and evolutionary origins of extraintestinal pathogenic Escherichia coli (ExPEC)are so far only partially defined, in the present study 100 of APEC isolates and 202 of UPEC isolates were compared by their content of virulence genes and phylogenetic groups. The two groups showed substantial overlap in terms of their serogroups, phylogenetic groups, and virulence genotypes including their possession of certain genes associated with large transmissible plasmids of APEC. In a chicken challenge model, both UPEC U17 and APEC E058 had similar LD50, demonstrating that UPEC U17 had the potential to cause significant disease in poultry. To gain further information of the correlation and pathogenesis of UPEC and APEC, the in vivo expression of 152 specific genes of UPEC U17 and APEC E058 both in murine urinary tract infection (UTI) model and chicken challenge model were compared with that of them grown statically to exponential phase in rich medium, respectively. The results show that in the same model (murine UTI model or chicken challenge model), part of the T-tested genes of UPEC U17 and APEC E058 show the similar tendency of expression. Several iron-related genes were up-regulated in UTI model and/or chicken challenge model, revealing that iron acquisition is critical for E. coli to survive in blood or urinary tract. Based on these results, the potential for APEC to act as human UPEC or as a reservoir of virulence genes for UPEC should be considered. Further, this study compared the transcriptional profile of virulence genes among APEC and UPEC in vivo.

³ E-mail: gsong{at}yzu.edu.cn