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Identification of surface proteins involved in the adhesion of a probiotic Bacillus cereus strain to mucin and fibronectin

¹ ENITA de Bordeaux;
² IECB

ABSTRACT

In the present study, several Bacillus strains isolated from commercial probiotic preparations were identified at the species level, and their adhesion capabilities to three different intestinal surfaces (mucin, Matrigel and Caco-2 cells) were assessed. In general, adhesion of spores was higher than that of vegetative cells to the three matrices, and globally strain Bacillus cereusCH displayed the best adhesion. Different biochemical treatments revealed that B. cereusCH surface proteins were involved in the adhesion properties of the strain. Surface-associated proteins from B. cereusCH vegetative cells and spores were extracted and identified, and some proteins such as S-layer, flagellin and cell bound proteases were found to bind to mucin or fibronectin. These facts suggest that those proteins might play important roles in the interaction of this probiotic Bacillus strain within the human gastrointestinal tract.

³ E-mail: m-urdaci{at}enitab.fr