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A Francisella novicida pdpA mutant exhibits limited intracellular replication and remains associated with the lysosomal marker LAMP-1

University of Victoria

ABSTRACT

Several genes contained in the Francisella Pathogenicity Island (FPI) encode proteins needed for intracellular growth and virulence of F. tularensis. The pdpA gene is the first cistron in the larger of the two operons found in the FPI. In this work we studied the intracellular growth phenotype of a F. novicida mutant in the pdpA gene. The pdpA strain was capable of a small amount of intracellular replication but, unlike wild type F. novicida, remained associated with the lysosomal marker LAMP-1, suggesting PdpA is necessary for progression from the early phagosome phase of infection. Strains with in cis complementation of the pdpA lesion showed a restoration of intracellular growth to wild type levels. Infection of macrophages with the pdpA mutant generated a host cell mRNA profile distinct from that generated by infection with wild type F. novicida. The transcriptional response of the host macrophage indicates that PdpA functions directly or indirectly to suppress macrophage ability to signal via growth factors, cytokines, and adhesion ligands.

¹ E-mail: fnano{at}uvic.ca

This article has been cited by other articles:

				C. L. Schmerk, B. N. Duplantis, D. Wang, R. D. Burke, A. Y. Chou, K. L. Elkins, J. S. Ludu, and F. E. Nano Characterization of the pathogenicity island protein PdpA and its role in the virulence of Francisella novicida Microbiology, May 1, 2009; 155(5): 1489 - 1497. [Abstract] [Full Text] [PDF]