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Published online ahead of print on 16 April 2009 as doi:10.1099/mic.0.026278-0
Microbiology 2009;155:1580.

Microbiology (2009), DOI 10.1099/mic.0.026278-0
© 2009 Society for General Microbiology
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Microbiology 0 (2009), mic.0.026278; DOI  10.1099/mic.0.026278-0
© 2009 Society for General Microbiology

Protective capacities of cell surface-associated proteins of Streptococcus suis mutants deficient in divalent cation-uptake regulators

J. Aranda1, M. E. Garrido1, N. Fittipaldi2, P. Cortés1, M. Llagostera1, M. Gottschalk3 and J. Barbé1,4

1 Universitat Autònoma de Barcelona;
2 Université de Montréal;
3 Universitéde Montréal

ABSTRACT

Many cell surface-associated, divalent-cation-regulated proteins are immunogenic and some of them confer protection against the bacterial species from which they are derived. In this work, two Streptococcus suis divalent-cation-uptake-regulator genes controlling zinc/manganese- and iron-uptake (adcR and fur, respectively) were inactivated in order to study the protective capacities of their cell surface-associated proteins. Results obtained showed an overexpression of a set of immunogenic proteins (including members of the Pneumococcal histidine triad family previously reported to confer protection against streptococcal pathogens) in the S. suis adcR mutant cell surface extracts. Likewise, genes encoding zinc transporters, putative virulence factors, and a paralog ribosomal protein related to zinc starvation, appeared derepressed in this mutant strain. Moreover, protection assays in mice showed that although neither adcR- nor fur-regulated cell surface-associated proteins were sufficient to confer protection in mice, the combination of both adcR- and fur-regulated cell surface-associated proteins are able to confer a significant protection (50%, P = 0.038) against a challenge to mice vaccinated with them.

4 E-mail: jordi.barbe{at}uab.es






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