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Published online ahead of print on 16 April 2009 as doi:10.1099/mic.0.026500-0
Microbiology 2009;155:1523.

Microbiology (2009), DOI 10.1099/mic.0.026500-0
© 2009 Society for General Microbiology
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Microbiology 0 (2009), mic.0.026500; DOI  10.1099/mic.0.026500-0
© 2009 Society for General Microbiology

The high-affinity phosphate transporter Pst in Proteus mirabilis HI4320 and its importance in biofilm formation

G. A. O'May1, S. M. Jacobsen1, M. Longwell2, P. Stoodley2, H. L. T. Mobley3 and M. E. Shirtliff4,5

1 University of Maryland - Baltimore Dental School;
2 Allegheny-Singer Research Institute;
3 University of Michigan Medical School;
4 U of Maryland, Baltimore

ABSTRACT

Proteus mirabilis causes urinary tract infections (UTIs) in individuals requiring long-term in-dwelling catheterization. The pathogenesis of this uropathogen is mediated by a number of virulence factors and formation of crystalline biofilms. In addition, microorganisms have evolved complex systems for acquisition of nutrients including the phosphate-specific transport system, which has been shown to be important in biofilm formation and pathogenesis. A functional Pst system is important during UTIs caused by P. mirabilis HI4320 since transposon mutants in the PstS periplasmic binding protein and the PstA permease protein were attenuated in the CBA mouse model of UTI. These mutants displayed a defect in biofilm formation when grown in human urine. This study focuses on a comparison of the proteomes during biofilm and planktonic growth in phosphate-rich medium and human urine, and microscopic investigations of biofilms formed by the pst mutants. Our data suggest that (i) the pst mutants - and particularly the pstS mutant - are defective in biofilm formation and (ii) the proteomes of these mutants differ significantly from the wild-type. Therefore, since the Pst system of P. mirabilis HI4320 negatively regulates biofilm formation, this system is important for the pathogenesis of these organisms during complicated UTIs.

5 E-mail: mshirtliff{at}umaryland.edu






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