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An iron-regulated LysR-type element mediates antimicrobial peptide resistance and virulence in Yersinia pseudotuberculosis

¹ Université Lille2;
² Institut Pasteur, Paris;
³ University of British Columbia;
⁴ Université Lille 2

ABSTRACT

During the course of its infection of the mammalian digestive tract, the entero-invasive, Gram-negative bacterium Yersinia pseudotuberculosis must overcome various hostile living conditions (notably, iron starvation and the presence of antimicrobial compounds produced in situ). We have previously reported (Marceau et al., 2004) that in vitro bacterial growth during iron deprivation raises resistance to the antimicrobial peptide polymyxin B; herein, we show that this phenotype is mediated by a chromosomal gene (YPTB0333) encoding a transcriptional regulator from the LysR family. We determined that YPTB0333's product is a pleiotropic regulator which controls (in addition to its own expression) genes encoding the Yfe iron uptake system and polymyxin B resistance. Lastly, by using a mouse model of oral infection, we demonstrated that YPTB0333 is required for colonization of Peyer's patches and mesenteric lymph nodes by Y. pseudotuberculosis.

⁵ E-mail: michael.marceau{at}ibl.fr