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Published online ahead of print on 21 April 2009 as doi:10.1099/mic.0.026799-0
Microbiology 2009;155:1800.

Microbiology (2009), DOI 10.1099/mic.0.026799-0
© 2009 Society for General Microbiology
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Microbiology 0 (2009), mic.0.026799; DOI  10.1099/mic.0.026799-0
© 2009 Society for General Microbiology

Phosphate and carbon source regulation of two PhoP-dependent glycerophosphodiester phosphodiesterase genes of Streptomyces coelicolor

F. Santos-Beneit1, A. Rodríguez-García2, A. K. Apel1 and J. F. Martín3,4

1 Instituto de Biotecnología de León, INBIOTEC;
2 Instituto de Biotecnología de León, INBIOTEC and Universidad de León;
3 Universidad de León & Instituto de Biotecnología de León, INBIOTEC

ABSTRACT

Glycerophosphodiesters are formed by deacylation of phospholipids. Streptomyces coelicolor and other soil dwelling actinomycetes utilize glycerophosphodiesters as phosphate and carbon sources by the action of glycerophosphodiester phosphodiesterases (GDPD). Seven genes encoding putative GDPDs occur in the S. coelicolor genome. Two of these genes, glpQ1 and glpQ2, encoding extracellular GDPDs, showed a PhoP-dependent up-regulated profile in response to phosphate shiftdown. Expression studies using the luxAB genes as reporter confirmed the PhoP dependence of both glpQ1 and glpQ2. Footprinting analyses with pure GST-PhoP of the glpQ1 promoter revealed four protected direct repeat units (DRu). PhoP binding affinity to the glpQ2 promoter was lower and revealed a protected region containing five DRu. As expected for pho regulon genes, inorganic phosphate and, also glycerol-3-phosphate, inhibited the expression from both glpQ1 and glpQ2. The expression of glpQ1 gene was also repressed by serine and inositol but expression of glpQ2 was not. On the contrary, glucose, fructose and glycerol increased expression of glpQ2 but not that of glpQ1. In summary, our results suggest an interaction of phosphate control mediated by PhoP and carbon source regulation of the glpQ1 and glpQ2 genes involving complex operator structures.

4 E-mail: jf.martin{at}unileon.es






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