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This Article Full Text (Papers in Press[PDF]) All Versions of this Article: mic.0.027409-0v1 155/6/2058    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ishii, Y. Articles by Namba, S. Search for Related Content PubMed PubMed Citation Articles by Ishii, Y. Articles by Namba, S. Agricola Articles by Ishii, Y. Articles by Namba, S.

In the non-insect-transmissible line of onion yellows phytoplasma (OY-NIM), the plasmid-encoded transmembrane protein ORF3 lacks the major promoter region

¹ Laboratory of Plant Pathology, The University of Tokyo;
² Department of Clinical Plant Science, Faculty of Bioscience and Applied Chemistry, Hosei University;
³ Laboratory of Clinical Plant Science, The University of Tokyo

ABSTRACT

'Candidatus Phytoplasma asteris', onion yellows strain (OY), a mildly pathogenic line (OY-M), is a phytopathogenic bacterium transmitted by Macrosteles striifrons leafhoppers. OY-M contains two types of plasmids (EcOYM and pOYM), each of which possesses a gene encoding the putative transmembrane protein, ORF3. Likewise, a non-insect-transmissible line (OY-NIM) has the corresponding plasmids (EcOYNIM and pOYNIM), but pOYNIM lacks orf3. Here we showed that, in OY-M, orf3 was transcribed from two putative promoters and that, on EcOYNIM, one of the promoter sequences was mutated and the other deleted. We also showed that ORF3 was not expressed in the OY-NIM-infected plants by immunohistochemical analysis. Moreover, ORF3 protein seemed more specifically expressed in OY-M-infected insects rather than in plants. We speculate that ORF3 might play a role in the interactions of OY with its insect host.

⁴ E-mail: anamba{at}mail.ecc.u-tokyo.ac.jp