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Published online ahead of print on 30 April 2009 as doi:10.1099/mic.0.027706-0
Microbiology 2009;155:2116.

Microbiology (2009), DOI 10.1099/mic.0.027706-0
© 2009 Society for General Microbiology
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Microbiology 0 (2009), mic.0.027706; DOI  10.1099/mic.0.027706-0
© 2009 Society for General Microbiology

Actinomyces naeslundii in initial dental biofilm formation

Irene Dige1,3, Merete K Raarup2, Jens Randel Nyengaard2, Mogens Kilian2 and Bente Nyvad2

1 School of Dentistry, Aarhus University, Denmark;
2 Aarhus University, Denmark

ABSTRACT

The combined use of Confocal Laser Scanning Microscopy (CLSM) and Fluorescent In Situ Hybridization (FISH) offers new opportunities for analysis of the spatial relationships and temporal changes of specific members of the microbiota of intact dental biofilms. The purpose of this study was to analyse the patterns of colonization and population dynamics of Actinomyces naeslundii compared to streptococci and other bacteria during the initial 48 h of biofilm formation in the oral cavity. Biofilms developed on standardized glass slabs mounted in intra-oral appliances worn by ten individuals for 6, 12, 24, and 48 h. The biofilms were subsequently labelled with probes against A. naeslundii (ACT476), streptococci (STR405), or all bacteria (EUB338) and were analysed by CLSM. Quantification of labelled bacteria was done by stereological tools. The results showed a notable increase in the number of streptococci and A. naeslundii over time with at tendency towards a slower growth rate for A. naeslundii compared with streptococci. A. naeslundii was located mainly in the inner part of the multilayered biofilm indicating that it is one of the species that attaches directly to the acquired pellicle. The participation of A. naeslundii in the initial stages of dental biofilm formation may have important ecological consequences.

3 E-mail: idige{at}odont.au.dk


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Oral bacterial biofilms – history in progress
Robert J. Palmer
Microbiology 2009 155: 2113-2114. [Full Text] [PDF]



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R. J. Palmer
Oral bacterial biofilms - history in progress
Microbiology, July 1, 2009; 155(7): 2113 - 2114.
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