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Published online ahead of print on 23 April 2009 as doi:10.1099/mic.0.028001-0
Microbiology 2009;155:2148.

Microbiology (2009), DOI 10.1099/mic.0.028001-0
© 2009 Society for General Microbiology
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Microbiology 0 (2009), mic.0.028001; DOI  10.1099/mic.0.028001-0
© 2009 Society for General Microbiology

Pseudomonas aeruginosa extracellular products inhibit staphylococcal growth and disrupt established biofilms by Staphylococcus epidermidis

Zhiqiang Qin1,4, Liang Yang2, Di Qu3, Soeren Molin2 and Tim Tolker-Nielsen2

1 Medical University of South Carolina;
2 Technical University of Denmark;
3 Shanghai Medical School of Fudan University

ABSTRACT

Multiple bacterial species often coexist as communities and compete for environmental resources. Here, we describe how an opportunistic pathogen Pseudomonas aeruginosa uses extracellular products to interact with the nosocomial pathogen Staphylococcus epidermidis. Both S. epidermidis biofilms and planktonic cultures were challenged with P. aeruginosa supernatant cultures overnight. Results indicate that quorum-sensing controlled factors from P. aeruginosa supernatant inhibited S. epidermidis growth in planktonic cultures. We also found that P. aeruginosa extracellular products, mainly polysaccharides, disrupted established S. epidermidis biofilms. Cellulase treated P. aeruginosa supernatant and supernatant from pelA, pslF and pelApslBCD mutants, which are deficient in polysaccharide biosynthesis, diminished the disruption of S. epidermidis biofilms. In contrast, S. epidermidis supernatant in overnight cultures has no effect on established P. aeruginosa biofilms and planktonic growth. These findings reveal that P. aeruginosa extracellular products are important microbial competition factors that overcome competition with S. epidermidis and they may provide clues for the development of a novel strategy for controlling S. epidermidis biofilms.

4 E-mail: qinzh{at}musc.edu






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