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The GPI-modified proteins Pga59 and Pga62 of Candida albicans are required for cell wall integrity

¹ Institut Pasteur;
² University of Amsterdam

ABSTRACT

The fungal cell wall is essential in maintaining cellular integrity and plays key roles in the interplay between fungal pathogens and their hosts. The PGA59 and PGA62 genes encode two short and related glycosylphosphatidylinositol-anchored cell wall proteins and their expression has been previously shown to be strongly up-regulated when the human pathogen Candida albicans grows as biofilms. Using GFP fusion proteins, we have shown that Pga59 and Pga62 are cell wall-located, N- and O-glycosylated proteins. The characterization of C. albicans pga59/pga59, pga62/pga62 and pga59/pga59 pga62/pga62 mutants suggested a minor role of these two proteins in hyphal morphogenesis and that they are not critical to biofilm formation. Importantly, the sensitivity to different cell wall perturbing agents was altered in these mutants. In particular, simultaneous inactivation of PGA59 and PGA62 resulted in high sensitivity to Calcofluor White, Congo Red and Nikkomicin Z and in resistance to caspofungin. Furthermore, cell wall composition and observation by transmission electron microscopy indicated an altered cell wall structure in the mutant strains. Collectively, these data suggest that the cell wall Pga59 and Pga62 proteins contribute to cell wall stability and structure.

³ E-mail: denfert{at}pasteur.fr

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