f Tellurite reductase activity of nitrate reductase is responsible for the basal resistance of Escherichia coli to tellurite
- Authors: Cécile Avazéri, Raymond J. Turner, Jeanine Pommier, Joël H. Weiner, Gérard Giordano, André Verméglio
- Author for correspondence: André Verméglio. Tel: + 33 4 42 25 46 30. Fax: +33 4 42 25 47 01. e-mail: vermeglio@:DRA.CAD.CEA.FR
- Microbiology, April 1997 143: 1181-1189, doi: 10.1099/00221287-143-4-1181
- Subject: Biochemistry
- Published Online:
Tellurite and selenate reductase activities were identified in extracts of Escherichia coli. These activities were detected on non-denaturing polyacrylamide gels using an in situ methyl viologen activity-staining technique. The activity bands produced from membrane-protein extracts had the same RF values as those of nitrate reductases (NRs) A and Z. Tellurite and selenate reductase activities were absent from membranes obtained from mutants deleted in NRs A and Z. Further evidence of the tellurite and selenate reductase activities of NR was demonstrated using rocket immunoelectrophoresis analysis, where the tellurite and selenate reductase activities corresponded to the precipitation arc of NR. Additionally, hypersensitivity to potassium tellurite was observed under aerobic growth conditions in nar mutants. The tac promoter expression of NR A resulted in elevated tellurite resistance. The data obtained also imply that a minimal threshold level of NR A is required to increase resistance. Under anaerobic growth conditions additional tellurite reductase activity was identified in the soluble fraction on non-denaturing gels. Nitrate reductase mutants were not hypersensitive under anaerobic conditions, possibly due to the presence of this additional reductase activity.
© Society for General Microbiology 1997 | Published by the Society for General Microbiology
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