f Spore surface glycoproteins of Colletotrichum lindemuthianum are recognized by a monoclonal antibody which inhibits adhesion to polystyrene
- Authors: H. Bleddyn Hughes, Raffaella Carzaniga, Sarah L. Rawlings, Jonathan R. Green, Richard J. O'Connell
- Author for correspondence: Richard J. O'Connell. Tel: +44 1275 392181. Fax: +44 1275 394281. e-mail: email@example.com
- Microbiology, August 1999 145: 1927-1936, doi: 10.1099/13500872-145-8-1927
- Subject: Plant-Microbe Interactions
- Published Online:
Conidia (spores) of Colletotrichum lindemuthianum, a fungal plant pathogen causing bean anthracnose, adhere to the aerial parts of host plants to initiate the infection process. These spores possess a fibrillar' spore coat' as well as a cell wall. In a previous study a mAb, UB20, was raised that recognized glycoproteins on the spore surface. In this study UB20 was used to localize and characterize these glycoproteins and to investigate their possible role in adhesion. Glycoproteins recognized by UB20 were concentrated on the outer surface of the spore coat and, to a lesser extent, at the plasma membrane/cell wall interface. Extraction of spores with hot water or 0-2% SDS resulted in removal of the spore coat. Western blotting with UB20 showed that a relatively small number of glycoproteins were extracted by these procedures, including a major component at 110 kDa. Biotinylation of carbohydrate moieties, together with cell fractionation, confirmed that these glycoproteins were exposed at the surface of the spores. In adhesion assays, < 90% of ungerminated conidia attached to polystyrene Petri dishes within 30 min. UB20 IgG at low concentrations inhibited attachment in an antigen-specific manner. This suggests that the glycoproteins recognized by this mAb may function in the initial rapid attachment of conidia to hydrophobic substrata. Polystyrene microspheres bound selectively to the 110 kDa glycoprotein in Western blots, providing further evidence that this component could mediate interactions with hydrophobic substrata.
Abbreviations: endo-H, endo-β-N-glucosaminidase-H; PA/TCH/SP, periodic acid/thiocarbohydrazide/silver proteinate; PNGase, peptide-N-glycosidase; TCS, tissue culture supernatant.
© Society for General Microbiology | Published by the Society for General Microbiology
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