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Front cover illustration

Scanning electron microscopy reveals bacterial interactions with epithelial cells. The upper image depicts commensal segmented filamentous bacteria selectively colonizing the epithelium overlying Peyer's patches in mouse intestine. The lower image shows, at higher magnification, Salmonella infecting a cultured epithelial cell. Commonly used cell lines do not fully reflect the properties of the specialized intestinal epithelium so Martinez-Argudo & Jepson (this issue, pp. 3887–3894) have re-examined Salmonella infection using a more sophisticated in vitro technique in which polarized human enterocyte-like cells are co-cultured with lymphocytes and acquire properties of Peyer's patch epithelia. Images courtesy Mark Jepson, Department of Biochemistry and Wolfson Bioimaging Facility, University of Bristol.